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- Methode der synthetischen Biologie (de)
- fully artificial DNA production method based on solid-phase DNA synthesis (en)
- otrzymywanie długich łańcuchów DNA poza komórkami (pl)
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- 300.0 (dbd:second)
- The discovery of orthogonal att sites that are specific allowed for the developed of Multisite Gateway Cloning technology. This allows for multiple DNA fragments to be assembled, with the order of assembly directed by the att sites. (en)
- The MoClo assembly standard allows for Golden Gate constructs to be further assembled in subsequent tiers. In the example here, four genes assembled via tier 1 Golden Gate assembly are assembled into a multi-gene construct in a tier 2 assembly. (en)
- The Golden Braid assembly standard also builds on the first tier of Golden Gate assembly and assembles further tiers via a pairwise protocol. Four tier one destination vectors are assembled into two tier 2 destination vectors, which are then used as tier 3 entry vectors for the tier 3 destination vector. Alternating restriction enzymes are used. (en)
- The Gateway Cloning entry vectors must first be produced using a synthesised DNA fragment containing the required attB sites. Recombination with the donor vector is catalysed by the BP clonase mix and produces the desired entry vector with attL sites. (en)
- DNA parts to be used for BASIC assembly need to contain integrated prefix and suffix sequences . These contain BsaI restriction sites that will allow for the iP and iS linkers to be attached to the DNA part. Once the linkers are attached, the part is ready for assembly. (en)
- The desired construct is obtained by recombining the entry vector with the destination vector. In this case, the final construct involves the assembly of two DNA fragments of interest. The attL sites on the entry vector recombine with the attR sites on the destination vector. The lethal gene ccdB is lost from the destination vector, and any bacteria that uptakes the unwanted vector will die, allowing selection of the desired vector. (en)
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- Summary of the key Gateway Cloning technologies. (en)
- The MoClo and Golden Braid assembly standards are derivatives of the original Golden Gate assembly standard. (en)
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- BASIC Parts Preparation.png (en)
- Four Part BASIC Assembly Construct.png (en)
- Gateway BP Clonase.png (en)
- Gateway LR Clonase.png (en)
- Golden_Braid_assembly.png (en)
- MoClo_assembly.png (en)
- Multisite Gateway Assembly.png (en)
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- Artificial gene synthesis (en)
- تخليق اصطناعي للجينات (ar)
- Künstliche Gensynthese (de)
- 인공 유전자 합성 (ko)
- Синтез искусственных генов (ru)
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